Reput(Ab)le Cancer-Signaling Antibodies

13 Jun 2016

We’re validated. We’re guaranteed. We’re published. We create the antibodies most cited by the research community. Recognizing both the tremendous opportunities and the challenges facing cancer researchers, EMD Millipore is dedicated to developing and refining tools and technologies for the study of cancer. With EMD Millipore’s comprehensive portfolio of reagents and antibodies, researchers can count on dependable, high-quality solutions for analyzing all the hallmarks of cancer.

MILLIPLEX MAP Human Complement Panel 1 - Immunology Multiplex Assay

Merck

EMD Millipore’s MILLIPLEX® MAP Complement Panel 1 Magnetic Bead Panel is an 8-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma or culture supernatant samples: Complement 2, Complement C4B, Complement C5, Complement C5a, Complement C9, Adipson/Complement Factor D, Mannose-Binding Lecting (MBL) and Complement Factor I. MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior(e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility. Each MILLIPLEX® MAP panel and kit includes: Quality controls (QCs) provided to qualify assay performance Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency Optimized serum matrix to mimic native analyte environment Detection antibody cocktails designed to yield consistent analyte profiles within panel EMD Millipore’s MILLIPLEX® MAP Complement Panel 1 Magnetic Bead Panel is part of the most versatile system available for complement factor research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.

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High-Definition Erenna® Immunoassay System

Merck

The Erenna Immunoassay System, powered by Singulex's patented single-molecule detection technology, accelerates biomedical and translational research by accurately and reliably quantifying low-abundance biomarkers at or below sub-picogram concentrations. This unique high-definition technology enables distinct stratification of healthy and disease subjects through a broad dynamic range of over 4 logs, enhancing the clinical utility of biomarkers and allowing researchers to gain insights into drug safety and efficacy. The Erenna Immunoassay System includes fully equipped benchtop analytical instrumentation, integrated and prepared reagents for ultrasensitive immunoassays, and dedicated software to automatically analyze data and display detailed assay reports. The Erenna Immunoassay System uses a robust digital molecule-counting module to count photons, where ultra-sensitive fluorescence is measured in the presence of proprietary background reduction methodology. A large dynamic range of 4+ logs is obtained by combining single-molecule counting (low range) with photon counting (mid range) and total light measurements (high range).

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Monoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. Browse our peer-reviewed product directory to find monoclonal antibodies for research and clinical applications; compare products, check reviews, and get pricing directly from manufacturers.Secondary AntibodiesImmunoassayImmunoassays are laboratory tests that utilize antibody-antigen interactions to detect specific substances. They are widely used in diagnostics for detecting diseases, drug testing, and environmental monitoring. These assays offer rapid, sensitive, and precise detection of biomarkers, making them invaluable in clinical and research labs. Browse our peer-reviewed product directory to compare immunoassay systems, read user reviews, and get pricing directly from manufacturers.Cancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.
Reput(Ab)le Cancer-Signaling Antibodies