ResourceGeneral Lab

Reliable time-to-result with a high throughput PCR purification protocol using magnetic beads

18 Mar 2025

PCR purification is a critical step in molecular biology workflows, ensuring the removal of primers, nucleotides, and other contaminants that can interfere with downstream applications such as sequencing, cloning, and genotyping. Using magnetic bead-based purification simplifies the process by selectively binding DNA fragments while eliminating unwanted byproducts.

In this application note, INTEGRA demonstrates an efficient and scalable PCR purification protocol using MAGFLO™ PCR beads with the MAG module on VIAFLO 96 and VIAFLO 384. The automated workflow enables high-throughput processing of 96 samples simultaneously, improving consistency and reducing hands-on time.

VIAFLO 96 – Electronic 24 and 96 Channel Handheld Pipette

INTEGRA Biosciences

VIAFLO 96 increases productivity of sample preparation in microplates. It is used like a standard handheld pipette. It is hand-controlled but movements are supported by motors for an effortless workflow and precise targeting of the microplate wells. The working volume range of VIAFLO 96 can be quickly adapted to the application’s requirements by changing the pipetting heads. The 24 channel heads are available in the volume ranges 10-300 µl and 50-1250 µl and the 96 channel heads in the volume ranges 0.5-12.5 µl, 2-50µl, 5–125 µl, 10–300 µl and 50–1250 µl. VIAFLO 96 benefits from the innovative GripTip system. The special pipette tips, together with the unique tip fitting, form an integral system. It guarantees tips will not fall off during pipetting and provides a reliable sealing of every tip. VIAFLO 96 can be used for sample transfers from reservoir to plate, plate to plate or within plates. Features and Benefits:  • VIAFLO 96 is used like a handheld pipette and has an intuitive user interface, freeing staff from training time. • Pipetting heads can be changed for an optimal instrument adaption to different volume ranges, increasing accuracy and precision. • The innovative GripTip system provides a reliable sealing of every tip and guarantees that tips will never fall off, providing safe and reproducible assay preparation. • Various pipetting modes are available for increased productivity and application customization.

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VIAFLO 384 – Electronic 24, 96 and 384 Channel Handheld Pipette

INTEGRA Biosciences

INTEGRA Biosciences’ VIAFLO 384 increases productivity of sample preparation in microplates and supports 24, 96 and 384 channel pipetting heads. VIAFLO 384 is used like a standard handheld pipette. It is hand-controlled but movements are supported by motors for an effortless workflow and precise targeting of the microplate wells. With a choice of ten interchangeable pipetting heads, the unit quickly adapts to any 24, 96, 384, or even 1536 well pipetting application in a volume range of 0.5 μl to 1250 μl.  VIAFLO 384 benefits from the innovative GripTip system. The special pipette tips, together with the unique tip fitting, form an integral system. It guarantees tips will not fall off during pipetting and provides a reliable sealing of every tip. VIAFLO 384 can be used for sample transfers from reservoir to plate, plate to plate or within plates. VIAFLO 384 Features and Benefits: VIAFLO 384 is used like a handheld pipette and has an intuitive user interface, freeing staff from training time. Pipetting heads can be changed for an optimal instrument adaption to different volume ranges, increasing accuracy and precision. The innovative GripTip system provides a reliable sealing of every tip and guarantees that tips will never fall off, providing safe and reproducible assay preparation. Various pipetting modes are available for increased productivity and application customization.

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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PCRDNA Purification
Enhancing DNA purification for high-quality downstream applications