Reliable Purification of PCR Products with High Yield and Purity Using the MACHEREY-NAGEL NucleoSpin® 96 PCR Clean-up kit on a Freedom EVO® Platform

16 Dec 2014

PCR has become a standard molecular biology tool for biological and medical research laboratories, and is used for DNA cloning, sequencing and genetic testing. For most applications, PCR products need to be purified, and this is advantageous to perform in a high throughput format. Fully automating the PCR product purification process on a Freedom EVO workstation streamlines laboratory workflows, enabling rapid, reliable purification of 96 samples in 50 min.

Freedom EVO® with Dual Liquid Handling Arms

Tecan

Tecan’s Freedom EVO series of automated workstations offer unrivalled processing speed and flexibility thanks to a unique feature that allows two liquid handling (LiHa) arms to act completely independently. Each arm of eight variable-spacing pipetting tips can be adapted to handle high volumes, for example of cell culture media, as well as low volumes of precious reagents or samples. The dual LiHa arms can run in parallel with the robotic manipulator (RoMa) arm which moves labware around the worktable, and are fully compatible with Tecan’s extensive range of application options, including storage elements, stirrers and incubation, separation or reading devices. This capability is a great asset for a variety of applications, including hit picking, serial dilutions, consecutive sample transfer and other aspects of assay preparation, and can greatly enhance the liquid handling power of any laboratory.

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PCR AutomationPCR automation describes the process of using a thermocycler to complete the polymerase chain reaction (PCR) process quickly and efficiently.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.
Reliable Purification of PCR Products with High Yield and Purity Using the MACHEREY-NAGEL NucleoSpin® 96 PCR Clean-up kit on a Freedom EVO® Platform