ResourceLife Sciences
REAlease Fluorochrome Technology for Multiparameter Cell Sorting
13 Feb 2019Multiparameter immunofluorescent labeling is the method of choice for sorting of cell populations out of heterogeneous mixtures. However, downstream applications of isolated cells are usually limited since fluorescence channels and epitopes are blocked by the antibody fluorochrome conjugates utilized for the flow sorting experiment. Accordingly, antibody fluorochrome conjugates that would allow for a release of cell epitopes and a reuse of fluorescence detection channels after cell sorting might be of high interest for certain downstream applications.
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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.FluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.Cell SortingCell sorting isolates specific cell populations based on physical or molecular characteristics, essential in immunology, cancer research, and regenerative medicine. Techniques like flow cytometry improve accuracy and speed. Compare cell sorting instruments and solutions in our product directory.