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Rapid Separation of Ibuprofen, Ibuprofen- Related Compound C, and Valerophenone Using Advanced UHPLC and Sub-2 μm Solid Core Column Technologies
Rapid Separation of Ibuprofen, Ibuprofen- Related Compound C, and Valerophenone Using Advanced UHPLC and Sub-2 μm Solid Core Column Technologies
17 Jun 2015This application note demonstrates the advantages of using the Thermo Scientific™ Accucore™ Vanquish™ C18+ 1.5 μm UHPLC column and the Thermo Scientific Vanquish UHPLC system for the separation of ibuprofen, ibuprofen-related compound C, and valerophenone. The advanced capabilities of the Vanquish UHPLC system allow the Accucore Vanquish UHPLC columns to be operated at higher flow rates that enable development of rapid analytical methods while maintaining performance.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HPLC Method DevelopmentIt is important to develop and validate HPLC methods in order to identify, quantify or purify compounds of interest in the most efficient way. Effective method development ensures optimization, quality control and cost effectiveness.Drug TestingIt is essential to test the efficacy and toxicity of drugs at every stage of their development to ensure that a pharmaceutical product is fit for purpose and safe for use before reaching the stage of mass production and distribution.