ResourceLife Sciences
Rapid Selection of Adherent Cell Lines Using ClonePix Technology
2 Dec 2014Two adherent cell lines, one expressing green fluorescent protein and the other red fluorescent protein were used to evaluate the efficacy of the ClonePix 2 System with a range of adherent cell picking reagents of increasing stringency for use in cell culture. This application note presents a workflow for adherent cell picking using the ClonePix System in which distinct clones can be imaged with the specific fluorescence channels, analyzed and picked proficiently.
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Cell Adhesion AssaysCell adhesion assays are used to quantitate attachment and analyze the molecular mechanisms for extracellular matrix adhesion, cell migration and sensitivity to inhibitors. Find the best cell adhesion assay equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.