ResourceSeparations
Rapid Screening of Sialic Acids in Glycoproteins by HPAE-PAD
27 Oct 2014In this application note, sialic acids are determined in five representative glycoproteins by acid hydrolysis followed by HPAE-PAD. Sialic acid determination by HPAE-PAD using the Thermo Scientific Dionex CarboPac PA20 Fast Sialic Acid column is specific and direct, eliminating the need for sample derivatization after hydrolysis. The use of a disposable gold on polytetrafluoroethylene (PTFE) working electrode simplifies system maintenance while providing consistent response with a lifetime of four weeks.
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Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sialic AcidsGlycoproteins