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Rapid qPCR Analysis from Highly Inhibited Tissue and Blood Sample Extractions

Kapa Biosystems, Inc.

Rapid qPCR Analysis from Highly Inhibited Tissue and Blood Sample Extractions

21 Dec 2015

Enzymatic inhibition, caused by both sample and extraction carryover inhibitors, is a significant obstacle when optimizing crude sample workflows. KAPA PROBE FORCE qPCR Kits include a highly processive third-generation enzyme—engineered via directed evolution—which enables direct crude sample analysis without sample purification. This application note demonstrates that, in combination with KAPA Express Extract, KAPA PROBE FORCE offers a highly efficient and streamlined sample-to-Cq workflow solution for both low- and high-throughput genotyping and gene expression applications (<1.5 hr).

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KAPA PROBE FORCE qPCR Kits

Kapa Biosystems, Inc.

KAPA PROBE FORCE is a highly inhibitor resistant qPCR master mix that removes the need for DNA purification, enabling streamlined sample-to-Cq workflows. The master mix contains a third generation DNA polymerase evolved to overcome blood, tissue, and plant PCR inhibitors. Crude samples can now be analyzed with comparable accuracy, reproducibility, and sensitivity as purified DNA using KAPA PROBE FORCE.   Features:   Streamline sample-to-Cq workflows  in <1 hour. Eliminate the time and cost of sample purification by amplifying directly from crude samples. Analyze a wide range of sample types including  whole blood,  cells, mouse tails, FFPE, leaf, stem, seed, and soil. Generate accurate and reproducible results. Kits include a third-generation DNA polymerase, evolved for robust target amplification and detection. Enzyme maintains high reaction efficiency in the presence of PCR inhibitors for reliable data generation. Break through high levels of qPCR inhibitors. Achieve greater levels of sensitivity  for inhibited blood, tissue, and plant samples. Convert purified DNA assays to crude workflows without observable Cq delays. Multiplex crude samples efficiently. Accelerate genotyping analysis with single reaction allelic discrimination of crude DNA extracts. Maximize data collection from precious samples, increase throughput, and reduce costs.   Applications: GMO testing. Mouse transgenics. SNP genotyping. Food/water pathogen detection. Infectious disease research. Cancer research.

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PurificationPurification techniques are essential for isolating compounds of interest from complex mixtures, such as proteins, nucleic acids, and chemicals. These techniques are critical in biochemistry, drug development, and environmental studies. Explore purification systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Tissue ProcessingTissue processing involves preparing biological tissue samples for microscopic analysis, including fixation, embedding, and sectioning. This process is essential for histopathology and diagnostic applications. Explore tissue processing products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.qPCRQuantitative PCR (qPCR) is a molecular biology technique used to amplify and measure the amount of a specific DNA or RNA target. It plays a crucial role in gene expression analysis, pathogen detection, and quantifying DNA or RNA in various applications. Real-time PCR provides precise, quantitative results and is widely used in research, diagnostics, and clinical studies. Browse our peer-reviewed product directory to find the best qPCR solutions; compare products, check reviews, and get pricing directly from manufacturers.Gene ExpressionHigh ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.EnzymesBlood AnalysisThe analysis of blood is vital for many areas of life sciences and forensic investigations. Blood samples can be tested for a number of different reasons such as diagnosis, glucose levels, cholesterol and drug testing.Genotyping

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Rapid qPCR Analysis from Highly Inhibited Tissue and Blood Sample Extractions