SelectScience
Join Free
Accelerating Science
ResourceLife Sciences

Rapid proteomics sample preparation with on-column digestion

Tecan
19 Aug 2024

Bottom-up proteomics is widely used for protein identification and post-translational modification analysis. Tecan details the analysis of trypsin-digested bovine serum albumin (BSA) with on-column digestion using its Narrow Bore Extraction™ (NBE) columns, followed by peptide clean up by solid phase extraction (SPE) utilizing Atlas sorbent in the same column. Discover how using the Tecan Resolvex® A200 combined with the on-column digestion capabilities of the NBE columns enables a high throughput and automated method for the digestion and purification of proteolytic peptides.

Related products

Request Quote for All Products

Resolvex® A200 Automated ppSPE Workstation

Tecan

The Resolvex A200 provides fully automated ppSPE in 1 ml columns and 96-well plates. It uses proven and reliable syringe pump technology to precisely and accurately deliver up to 11 protocol solvents.

(3)

Links

TecanCompany website

Tags

Digestion and ExtractionDigestion and extraction apparatus are used in sample preparation to break down samples or extract organic or aqueous compounds from a solid. Digestion may be conducted via hot block, microwave or Kjeldahl digestion apparatus. Extraction systems typically involve solvent extraction and include Soxhlet, Randall or Twisselmann extraction methods. Find the best digestion and extraction apparatus in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Sample ProcessingSample processing is required to prepare samples prior to analysis. Technologies used for sample processing include homogenizers, grinders, mills and mixers.Bovine Serum Albumin (BSA)Solid Phase Extraction (SPE)Solid phase extraction (SPE) is a sample preparation technique used to separate and concentrate analytes from complex mixtures. It is widely used in environmental, pharmaceutical, and clinical analysis. SPE provides higher sensitivity and selectivity compared to traditional liquid-liquid extraction methods, making it essential for accurate and reproducible results. Explore SPE tools and systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

Related resources

Rapid proteomics sample preparation with on-column digestion