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Rapid Protein Precipitation without the Complications

Phenomenex Inc
7 Feb 2017

This application note describes Phenomenex’s protein precipitation plate capabilities to rapidly filter and precipitate a large number of samples simultaneously. This enables fast analysis, ensures that samples are clean and reduces the number of filtration steps.

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Impact™ Protein Precipitation Plates

Phenomenex Inc

Impact™ protein precipitation plates with Solvent Shielding Technology™ offer a rapid and convenient way to remove proteins from plasma and tissue homogenate samples prior to analysis.The Solvent Shielding Technology design will withhold organic solvents above the filter membranes for up to 25 minutes, allowing for direct in-well precipitation upon sample addition. The precipitate is then filtered out via vacuum, centrifuge or positive pressure resulting in a clean, protein depleted extract.

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FiltrationFiltration and microfiltration are used to separate solids, particulates or large molecules from fluids (liquid or gases) in order to purify the filtrate. Explore a range of filter formats, including filter paper, plates and membranes for your separation needs. While larger particulates may be separated by gravity filtration, smaller particulates may require additional force for timely separation. Microfiltration equipment includes vacuum filters, as well as positive pressure, crossflow and centrifugal filtration devices. Find the best filtration products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Plate ProcessingAutomated plate processing is used in microplate technologies for high-throughput, speed and efficiency. Plate processing systems include microplate handlers for moving plates, heat sealers, stackers and carousels for storage, retrieval and delivery, microplate washers and piercers. Useful features of plate processors include walk-away automation, compatibility and easy integration with microplate systems.Sample ProcessingSample processing is required to prepare samples prior to analysis. Technologies used for sample processing include homogenizers, grinders, mills and mixers.Tissue ProcessingTissue processing involves preparing biological tissue samples for microscopic analysis, including fixation, embedding, and sectioning. This process is essential for histopathology and diagnostic applications. Explore tissue processing products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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Rapid Protein Precipitation without the Complications