Rapid Protein Analysis and Screening of Membrane Protein Buffer Conditions by Gel Filtration

12 Jan 2011

This application note shows that protein-protein interaction studies are fast and easily carried out using Superdex 200 5/150 GL columns. Screening of buffer conditions for a membrane protein was performed within a few hours and with only 6×10 μl (68 μg) sample consumption.

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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.
Rapid Protein Analysis and Screening of Membrane Protein Buffer Conditions by Gel Filtration