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Rapid characterization of oligonucleotides using microfluidic capillary electrophoresis

908 Devices
26 Jun 2022

Oligonucleotides (oligos) represent a distinct class of therapeutics that include RNA, DNA, and their structural analogs, and are effective against a wide range of disease conditions. In this application note, 908 Devices presents a CE/ MS analysis of oligos by ZipChip coupled to a QExactive HF mass spectrometer. This positive ESI mode method utilizes an MS-friendly background electrolyte (BGE), that eliminates the use of ion-pairing agents such as hexafluoro isopropanol or alkyl amines.

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ZipChip™

Repligen Corp

ZipChip enables seamless CE/ESI-MS workflow and provides fast CE separation, nano-spray level sensitivity to obtain high-resolution separation and mass spec data from characterization of intact proteins, antibody drug conjugates (ADCs), antibody subunits, peptides, and nucleic acids. 

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.RNARNA is a nucleic acid that plays a key role in gene expression and protein synthesis. It serves as a messenger between DNA and ribosomes, carrying genetic instructions to produce proteins. Advances in RNA-based therapies, such as mRNA vaccines and gene editing, have revolutionized treatment strategies for genetic disorders and infectious diseases. Explore RNA research tools and therapies in our peer-reviewed product directory; compare products, check customer reviews, and get pricing directly from manufacturers.

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