Rapid Assessment of Size Changes Using the Scepter<sup>TM</sup>Cell Counter can be Predictive of Cell Death

13 Jun 2011

This application note describes a cell counting device used for the analysis of cells undergoing apoptosis. The ScepterTM handheld, automated cell counter from Millipore uses impedance-based particle detection to reliably and precisely count every cell in a sample. Precise volumes of single-cell suspensions are drawn into the sensor at the end of the Scepter device. Using NIH 3T3 and CHO cells, Millipore demonstrate that the relationship between apoptosis and cell volume depends on cell type and a specific apoptosis-inducing agent.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Plate ProcessingAutomated plate processing is used in microplate technologies for high-throughput, speed and efficiency. Plate processing systems include microplate handlers for moving plates, heat sealers, stackers and carousels for storage, retrieval and delivery, microplate washers and piercers. Useful features of plate processors include walk-away automation, compatibility and easy integration with microplate systems.
Rapid Assessment of Size Changes Using the Scepter<sup>TM</sup>Cell Counter can be Predictive of Cell Death