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Rapid and Simple Analysis of Dried Blood Spot Samples with a microLC/MS/MS Workflow

SCIEX
16 Jan 2015

The quantitation of drugs and endogenous small molecules in dried blood samples (DBS) is becoming increasingly important and micro-flow liquid chromatography has become a compelling alternative to conventional HPLC for many analyses, given its benefits of low sample consumption, high throughput and low solvent consumption. This poster presents the quantitative analysis of Phosphatidylethanol in DBS as an example. Analysis time was less than 2.5 minutes using a single channel micro LC.

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BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Toxicology / Drugs of Abuse TestingToxicology and Drugs of Abuse Testing is the investigation into toxic and illegal substances found at the scene of a crime or from a suspect and / or victim associated with a scene of a crime. Analyzers, immunoassays and drugs of abuse test kits and presumptive test kits are an essential aspect of toxicology investigation.Dried Blood Spots

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Rapid and Simple Analysis of Dried Blood Spot Samples with a microLC/MS/MS Workflow