Rapid and Sensitive Analysis of a 93-Compound Forensic Panel in Urine using the QTRAP/Triple Quad 4500 LC-MS/MS System
21 Aug 2017This technical note describes a rapid and sensitive analysis of a comprehensive panel of forensic compounds in human urine using the ExionLCTM AC HPLC system and the QTRAP®/Triple Quad™ 4500 LC-MS/MS system. This forensic panel contains 93 compounds, and a total of 212 MRM transitions (including internal standards) are monitored. Some compounds ionize preferentially in positive mode, while others ionize preferentially in negative mode; therefore this method takes advantage of rapid polarity switching. The total LC runtime is 6.5 minutes, which can be further accelerated for a smaller panel of compounds. Sample preparation is based on enzymatic hydrolysis and a simple “dilute and shoot” methodology.
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QTRAP 4500 system
SCIEXThe QTRAP 4500 system takes the legendary 4000 QTRAP platform and intelligently re-engineers it to set a new benchmark for reliable quantitation and library searching – with 100-fold more full-scan sensitivity over basic triple quadrupoles in the same class. The LC-MS/MS workhorse is intelligently re-engineered. As one of the only triple quadrupole instruments with optional Linear Accelerator Trap technology, the QTRAP 4500 system enables powerful, unique workflows that deliver a new level of confidence in your data. High-confidence library searching and fast eQ electronics for UHPLC: The 4500 series uses proven eQ electronics delivering polarity switching speeds of 50 msec and scan speeds of 20,000 Da/sec, creating a potent solution for detecting unexpected contaminants. Combined with a single comprehensive library of thousands of QTRAP spectra for library matching, contaminants at the lowest levels can be confidently identified on a UHPLC time scale. Features: Simultaneously quantitate MRMs and perform full scan library searches for non-targeted contaminants. Achieve a 100X increase in full-scan sensitivity over triple quads providing an enhanced level of confidence for forensic toxicology applications. Boost selectivity with quantitative MRM3 workflows – and reduce the need for extensive sample cleanup or labor-intensive chromatography methods. Obtain comprehensive peptide sequence confirmation and simplify MRM assay development for peptide quantitation.