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RAFT Culture Viability Determination Using the LIVE/DEAD Viability/Cytotoxicity Assay

TAP Biosystems
17 Jan 2013

In this application note the LIVE/DEAD Viability/Cytotoxicity kit from Life Technologies is shown to be an easy and rapid assay that can be used to assess cell viability in a RAFT 3D cell culture. Controls need to be included in the test to ensure that possible background noise is taken into account.

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RAFT (Real Architecture for 3D Tissue)

TAP Biosystems

RAFT is a new 3D cell culture system that enables scientists to create complex 3D tissue models in a simple, consistent and reproducible format. RAFT uses physiologically relevant collagen concentrations to create the most natural environment for cells. RAFT enables scientists to create 3D cell cultures in 3 simple steps in less than 1 hour. RAFT can be used to support assay development and secondary screening applications. It can also be used in a broad range of cell biology applications including: stem cell research, oncology research, toxicology and tissue engineering / scaffolds.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Cell ViabilityCell viability assays assess the health of cells and their ability to proliferate. These tests are crucial in drug development, toxicity testing, and cell-based research. Common methods for assessing viability include MTT, live/dead assays, and flow cytometry. Browse our peer-reviewed product directory to find the best tools for cell viability assays, compare products, check reviews, and get pricing directly from manufacturers.CytotoxicityCytotoxicity assays measure the toxic effects of substances on cells, often used in drug testing and environmental studies. These tests are crucial in determining the safety of chemicals and pharmaceutical compounds. Explore cytotoxicity testing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Cell AnalysisThe analysis of cells allows researchers to understand the factors which contribute to cell health and function. These influencing processes can then be predicted and altered, leading to the development of medication and disease treatments.

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RAFT Culture Viability Determination Using the LIVE/DEAD Viability/Cytotoxicity Assay