Rabbit monoclonal antibody development from ProSci

7 Dec 2023

Recombinant rabbit monoclonal antibodies are excellent tools for challenging targets such as small molecules or post-translational modifications. They have high affinity due to the rabbit’s robust immune system. Several methods are used to develop custom rabbit monoclonal antibodies, such as hybridoma generation, phage display, or B-cell cloning. This application note highlights ProSci’s B-cell development platform that allows for careful selection of the ideal antibodies and preservation and immortalization through conversion to a recombinant. The note also explores ProSci’s rabbit monoclonal antibody development service that offers two different packages: Premier – Client-provided splenocytes and Pro – Client-provided antigens, as well as customized packages.

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Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. 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Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Monoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. 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