ResourceLife Sciences
Quick and Simple Validation of Simple Plex Assays
Quick and Simple Validation of Simple Plex Assays
24 Sept 2015This application note demonstrates the process of evaluation of Simple Plex assays, and explains the validation process including running a calibration curve, analyzing replicates within a cartridge and between different cartridges, and spike and recovery experiments.
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Assay AssemblyAssay Assembly is technique used in drug discovery to develop assays to test the cytotoxicity, genotoxicity, or other activities of a compound on a cell. Assay assembly requires chip assembly, a delivery system and a detection and analysis method. Beneficial features of assay kits or automated systems include high-throughput, high speed and sensitivity and low signal to noise ratio.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Assay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.Method ValidationMethod validation is the process used to prove that the analytical procedure used for a particular test is capable of providing suitable data.Calibration