Qubit dsDNA assay specificity in the presence of ssDNA

1 Aug 2019

In this application note, Thermo Fisher Scientific investigates whether Qubit double-stranded DNA (dsDNA) quantitation assays accurately determine the concentration of dsDNA when the samples contain short or long single-stranded DNA (ssDNA).

Qubit™ dsDNA BR Assay Kit

Thermo Fisher Scientific

The Qubit dsDNA broad range assay kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentration from 100 pg/µl–1,000 ng/µl.

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Qubit™ 4 Fluorometer, with WiFi

Thermo Fisher Scientific

The Qubit 4 Flurometer with WiFi is the latest version of the popular Qubit fluorometer designed to accurately measure DNA, RNA, and protein quantity, and now also RNA integrity and quality, using the highly sensitive Qubit assays.

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DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.
Qubit dsDNA assay specificity in the presence of ssDNA