Quantitative Imaging of Chemiluminescent Western Blots: Comparison of Digital Imaging and X-ray Film
30 Jun 2015Chemiluminescent western blotting is the most frequently used method for detecting proteins. Recently there has been a trend towards chemifluorescence which provides the user with many advantages including shorter exposure times and also means the signal is stable for weeks. This application note will compare the performance of x-ray film with that of Syngene’s G:BOX Chemi IR6 imaging system for the visualisation and quantification of chemiluminescent and chemifluorescence signals on western blots.
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G:BOX Chemi XRQ
SyngeneG:BOX Chemi XRQ is a cost-efficient chemiluminescence imaging and gel documentation system. For a laboratory that needs hassle-free chemiluminescent detection, as well as routine gel documentation, using the G:BOX Chemi XRQ’s powerful GeneSys software to switch between applications is simplicity itself.
G:BOX Chemi XX6
SyngeneG:BOX Chemi XX6 gel imaging system has a high resolution camera for imaging multiple sample types and sizes, from fluorescence 1D to 2D gels to chemiluminescent blots. Your lab’s imaging system shouldn’t control how you detect proteins on Western blots. Chemiluminescence is great if you want sensitive detection of picogram or femtogram amounts, while fluorescence lets you quantify and detect multiple different proteins on one blot.
G:BOX Chemi XX9
SyngeneG:BOX Chemi XX9 gel imaging system has a high resolution camera for imaging multiple sample types and sizes, from fluorescence 1D to 2D gels to chemiluminescent blots. Your lab’s imaging system shouldn’t control how you detect proteins on Western blots. Chemiluminescence is great if you want sensitive detection of picogram or femtogram amounts, while fluorescence lets you quantify and detect multiple different proteins on one blot.