ResourceLife Sciences
Quantitation of Residual DNA in Final Product Biopharmaceutical using the Threshold Total DNA Assay System
10 Oct 2011In this application note by Molecular Devices, the precision, accuracy, and detection limit of the Threshold® Total DNA Assay was evaluated for the analysis of biopharmaceuticals products. Using the Threshold System and DNA Assay Kit, quantitation accuracy was assessed by evaluating the recovery of DNA added to an unknown sample relative to buffer controls treated in the same manner. Recovery experiments included sample pre-treatment steps to ascertain whether exogenous DNA was introduced while handling the sample. Suggestions are presented on how to determine both the quantity of residual DNA and the detection limit from biopharmaceutical products.
Links
Tags
Assay AssemblyAssay Assembly is technique used in drug discovery to develop assays to test the cytotoxicity, genotoxicity, or other activities of a compound on a cell. Assay assembly requires chip assembly, a delivery system and a detection and analysis method. Beneficial features of assay kits or automated systems include high-throughput, high speed and sensitivity and low signal to noise ratio.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Biopharmaceutical AdvancesBiopharmaceutical advances follow the development of pharmaceuticals derived from biotechnology, also known as biotechnology medicines. Biopharmaceuticals may be produced from cell lines, plants, or microbial cells. Important considerations of biopharmaceutical use include application, cost, production process and purification.