ResourceSpectroscopy
Quantitation of Protein Using the Micro-Volume BioSpec-nano Spectrophotometer
6 Nov 2012In this application note the BioSpec-nano spectrophotometer from Shimadzu demonstrates the ability to quantitatively measure the amount of protein in a sample. Using the 0.2 mm path length and 0.7 mm path length, the detection limits for protein analysis were calculated to be 0.149 mg/mL and 0.018 mg/mL respectively. Protein analysis is commonly carried out in the life sciences laboratories. The need for a rapid and simple analysis tool for protein samples is met with the use of the micro-volume BioSpec-nano. The included software for the BioSpec-nano provides the instrumental capabilities needed for protein analysis in the modern laboratory.
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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.UV-Visible SpectroscopyUltraviolet-visible (UV-Vis) spectrophotometers are used to measure the interaction of UV and visible light with a sample, including transmission, reflectance & absorbance. The two major instrument classes are single-beam or double-beam spectrophotometers. More specialized equipment includes colorimeters, spectroradiometers and refractometers. Portable and microvolume spectrophotometers are also available. For the modular spectroscopy lab, explore a range of light sources for combination with a spectrograph/spectrometer and optics. Find the best UV-Vis spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein QuantificationThe detection and quantification of proteins in a sample is vital across life sciences, pharmaceutical research and clinical diagnostics, and a variety of equipment is available to scientists to simplify the workflow. Proteins of interest can be easily labeled and detected on light-based detection instruments. Immunoassay kits allow you to identify a specific protein of interest and protein detection beads or antibody microarrays allow you to identify multiple specific proteins at once. Protein interactions and enzyme activity can also be monitored with protein-protein interaction assays. Additionally, the biophysical characterization of proteins is made easy with biokinetic analyzers. Find the best protein detection and quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.