ResourceSpectroscopy
Quantitation by High Resolution Mass Spectrometry: Using Target Enhancement and Tof-MRM to Achieve Femtogram-level On-column Sensitivity for Quantitation of Drugs in Human Plasma
8 Oct 2014In this application note the SYNAPT G2-S instrument is used to determine and compare the quantification attributes of detection limit (LOD), quantification limit (LOQ), and linear dynamic range for three data-acquisition modes: full-scan MS, MSE and Tof-MRM. In the presence of human plasma, the Tof-MRM mode of data acquisition can achieve on-column limits of detection that are in the low-femtogram range. Tof-MRM has direct applicability for bioanalysis, targeted metabolism, stability, and PK/PD profiling studies with low detection limits.
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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer.
Human PlasmaHuman plasma is the liquid component of blood, which carries blood components - nutrients, hormones, proteins, and waste - throughout the body. Plasma-based studies provide insights into biomarkers, immune responses, and disease mechanisms. Compare plasma handling tools and systems in our peer-reviewed product directory.
