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Quantitating DNA from FFPE Tissue: Superior sensitivity of the QuantiFluor dsDNA System versus the NanoDrop Spectrophotometer

Promega Corp.
19 Nov 2013

Accurate quantitation of DNA concentration is critical for many applications. Traditional spectrophotometric assays cannot determine DNA concentrations below 2 ug/ml; however, many isolated DNA samples have concentrations well below this level. This study comparatively examines the performance of the QuantiFluor™ dsDNA System with regards to accuracy and sensitivity to sample concentrations.

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QuantiFluor® dsDNA System

Promega Corp.

The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution.   The quantitation of dsDNA is a very important step in many biological applications, particularly in standard molecular biology techniques. The dye shows minimal binding to single stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA. The QuantiFluor® dsDNA System was developed using the fluorescence module of the GloMax® Multi+ Detection System and the QuantiFluor®-ST Handheld Fluorometer. The QuantiFluor® dsDNA System can be used with any fluorometer that is capable of measuring fluorescence at the appropriate excitation and emission wavelengths, including the Quantus™ Fluorometer. Each QuantiFluor® dsDNA System contains sufficient volume to perform 200 assays at 2ml or 2,000 assays at 200μl. QuantiFluor® dsDNA System Features & Benefits:   Specificity - Highly specific to dsDNA, minimal binding to ssDNA, RNA, protein and interfering compounds. Sensitivity - Significantly increased sensitivity over absorbance at 260nm (NanoDrop® spectrophotometer) for low-concentration samples. Performs better or equal to PicoGreen® dye and can detect as little as 50pg/ml. Ease of Use - System includes all required reagents to quickly set up and quantitate dsDNA. Instrument Compatibility - Pre-optimized on both the Quantus™ Fluorometer and GloMax®-Multi+ Instrument.

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