Quantifying the stoichiometry of protein–protein interactions

26 Mar 2020

In this application note, Fluidic Analytics demonstrates how microfluidic diffusional sizing (MDS) using the Fluidity One-W can be used to quantify the stoichiometry of protein–protein interactions.

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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Biopharmaceutical AdvancesBiopharmaceutical advances follow the development of pharmaceuticals derived from biotechnology, also known as biotechnology medicines. Biopharmaceuticals may be produced from cell lines, plants, or microbial cells. Important considerations of biopharmaceutical use include application, cost, production process and purification.MicrofluidicsMicrofluidics is the science of manipulating small volumes of fluids in micro-sized channels. It is widely used in diagnostics, drug development, and lab-on-a-chip technologies. Microfluidic devices can enable rapid, cost-effective, and high-throughput analysis of biological samples. Browse our peer-reviewed product directory to find the best microfluidic devices, compare products, check reviews, and get pricing directly from manufacturers.Protein InteractionsProtein interactions are essential for understanding cellular processes, as proteins work together to carry out biological functions. Studying protein interactions can provide insights into disease mechanisms, drug development, and cell signaling pathways. Explore the best tools for protein interaction research in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Protein StructureProtein structure refers to the three-dimensional arrangement of amino acids in proteins, determining their function. Understanding protein structure is key in drug discovery, enzymology, and molecular biology. Explore protein structure analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.
Quantifying the stoichiometry of protein–protein interactions