SelectScience
Join Free
Accelerating Science
ResourceLife Sciences

Quantifying Cross-Contamination from Tissue Homogenization with a Rotor-Stator Homogenizer Equipped with a Stainless Steel Generator Probe

OMNI International Inc.
19 Oct 2014

In research labs, rotor-stator homogenizers are critical pieces of equipment as they are routinely used as the first step in many experimental workflows. While rotor-stator homogenizers are extremely effective, between samples, researchers must clean the generator probe to remove sample particulate in order to prevent cross contamination between samples. The generator probe must be fully disassembled, cleaned and autoclaved prior to the next sample being processed. As an alternative to stainless steel generator probes, Omni International offers a disposable plastic Omni Tip™ generator probe that can be disposed of after each use to ensure that no cross contamination will occur. Herein, the effectiveness of standard generator probe cleansing processes to quantify the degree of DNA and protein carryover from sample to sample using a standard stainless steel generator probe has been evaluated.

Related products

Request Quote for All Products

Omni Tip Homogenizer Kit

OMNI International Inc.

Omni Tip™ Homogenizing Kits include everything you need to homogenize. These kits can be used to process sample sizes from 0.25mL to 30mL with Omni Tips & Omni Tip Kits come complete with the redesigned Omni TH Motor, patented Omni Tip™ Plastic Homogenizer Probes (in hard or soft tissue versions), Omni Tip™ Adapter, and tool kit all in a convenient storage case. Patented Omni Tip™ Plastic Homogenizer Probes are designed to completely avoid cross-contamination between samples during homogenization. Engineered from a durable plastic, they come in two different configurations for soft tissue and hard tissue homogenizing. Omni Tips are available in a standard size of 7mm (Diameter) x 110mm (Length).The outer tube of Omni Tips is made of Lexan 144R, a polycarbonate.The inner shafts are made of Ultem 1000, a polyetherimide. The Omni Tip Kit is extremely cost effective and will handle a wide range of applications for multiple users making it the perfect homogenizer solution for a small laboratory. Ideal for PCR, RNA, and DNA sample preparation along with environmental, pharmaceutical, microbiological research and soft or hard (frozen) tissue analysis Omni Tips™ are economical and can be disposed of after use or autoclaved up to 7 times No need to order multiple items, everything is contained in one package Omni TH Motor has a variable speed controller that allows for speeds of 5,000 to 35,000 rpm Kits are also available with 5mm, 7mm, or 10mm stainless steel generator probes for more fibrous/tough samples Includes an Omni TH Motor, Omni Tip™ Adapter, 12 Soft or 12 Hard Tissue Omni Tips™, stand clamp, convenient storage case and instruction manual.

(1)

Links

OMNI International Inc.Company website

Tags

Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Homogenizers and BlendersHomogenizers and blenders are used to homogenize various types of material, including tissue, cells, plants, food, soil, biological and chemical samples for subsequent analysis. Lab equipment for sample homogenization includes handheld and benchtop homogenizers, bead mill, ultrasonic and multiple sample homogenizers, high shear homogenizers and blenders. Homogenizer bead and probe multipacks are available to avoid cross contamination of samples, as well as cooling systems for homogenizing temperature-sensitive samples. Homogenizers are often used in the first step of extraction of DNA, RNA and proteins from tissue and cell samples. Find the best homogenizers and blenders in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Tissue ProcessingTissue processing involves preparing biological tissue samples for microscopic analysis, including fixation, embedding, and sectioning. This process is essential for histopathology and diagnostic applications. Explore tissue processing products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Sample HomogenizerSample ProcessingSample processing is required to prepare samples prior to analysis. Technologies used for sample processing include homogenizers, grinders, mills and mixers.

Related resources

Quantifying Cross-Contamination from Tissue Homogenization with a Rotor-Stator Homogenizer Equipped with a Stainless Steel Generator Probe