ResourceLife Sciences
Quantification of Total Nucleic Acids from Unknown Sources (DNA eq)
1 Jan 2018In this note, we describe how to use the Total Nucleic Acids application on the Lunatic systems. This Unmix application is used to analyze the UV/Vis spectral shape of the sample to isolate the fraction of the molecule of interest from co-absorbing entities contributing to the total UV/Vis absorption spectrum. Accurate quantification of the molecule of interest is established using its isolated spectrum fraction. This application does not distinguish DNA from RNA, but rather isolates the total nucleic acid concentration in DNA extractions from unknown sources (bacterial, yeast, plant, plasmid, etc.).
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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA QuantificationDNA quantification is the process of measuring the amount of DNA in a sample. It is vital in genomics, molecular biology, and biotechnology, helping to ensure accurate experiments and data analysis. Explore DNA quantification tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.