ResourceLife Sciences
QIAGEN® PLasmid Purification Technologies for High-Throughput Transfection Screening Using Automated and Manual Processing
29 Nov 2013Different manual and automated purification technologies were examined to study the effects of DNA quantity and purity on transfection efficiency. Human cells (293 and Huh-7) and Chinese Hamster Ovary cells (CHO-K1) were transfected with plasmid DNA isolated using either MagAttract® 96 miniprep technology on a BioRobot® 8000 workstation, QIAprep® 96 Turbo technology on a BioRobot 3000 workstation, or QIAwell® Ultra technology using a manual procedure.
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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.TransfectionTransfection introduces nucleic acids (DNA or RNA) into cells, facilitating gene expression, genome editing, and functional studies. It�s widely used in genetic research, drug discovery, and therapeutic development. Find transfection reagents, kits, and systems in our product directory with reviews and pricing options.