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Purine nucleotides analyzed with HPLC

MicroSolv Technology Corp.
23 Mar 2022

In this application note, a method is shown that achieves the separation of Nucleotides XMP from GTP. The analysis of these polar compounds is achieved at a high concentration of an organic Solvent as part of the Mobile Phase which provides increased Sensitivity. Retention times were very Reproducible with a %RSD of approximately 0.4, even when Red Blood Cell extracts were injected in between the standard samples.

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Cogent Diamond Hydride™ HPLC Columns

MicroSolv Technology Corp.

Cogent Diamond Hydride HPLC Columns are manufactured with TYPE-C™ silica (silica hydride) and a very small amount of carbon on the surface that adjusts the column hydrophobicity to an important level. This makes these columns have unique separation capabilities for scientists working in LCMS, metabolomics, bioanalytical, amino acids, organic acids, carbohydrates, and very polar small molecules.

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