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Purification of High-quality RNA from Cell and Tissue Samples with the Thermo Scientific KingFisher Pure RNA Tissue Kit

23 Jan 2015

This technical note describes total RNA purification from several mammalian cell lines, various tissue samples, and microbial cultures with the Thermo Scientific™ KingFisher™ Pure RNA Tissue Kit. The purified total RNA has high integrity and is free of contaminants or inhibitors, thus providing pure RNA for direct use in various downstream applications.

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Thermo Scientific KingFisher Pure Kits

Thermo Fisher Scientific

The Thermo Scientific KingFisher Pure Kits are specially designed for use with the KingFisher nucleic acid purification system. These optimized reagent kits help you achieve rapid, high-quality results for a variety of applications – with minimal effort. KingFisher Pure Kits are now available for Cell and Tissue DNA, DNA Blood, DNA Plant, Plasmid, RNA Blood, RNA Plant, RNA Tissue, and Viral NA.Key Features: Developed specifically for use with KingFisher magnetic particle processors Optimized for DNA/RNA purification from a variety of sources Easy-to-use system for pure performance, every time Application Areas: Genetic testing Microbial identification Plant genetics Evolutionary studies / Phylogenetics

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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