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Purification of DMT-on oligonucleotides using HIC resins
16 Jul 2024Dimethoxytrityl (DMT), a 5’ protecting group, is used in the synthesis of oligonucleotides to temporarily mask the characteristic chemistry of a 5’-hydroxy functional group. In many preparations of delicate oligonucleotides, DMT may be left on an oligonucleotide following synthesis to give stability to the molecule during subsequent processing. Tosoh Bioscience highlights a novel, effective and high recovery method for the purification of a DMT-on oligonucleotide. The effective removal of a DMT-group from an oligonucleotide in a single purification step is also described. Discover how hydrophobic interaction chromatography (HIC) can be applied in the purification process, using TOYOPEARL PPG-600M, Phenyl-650M and Butyl-650M HIC resins.
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Preparative ChromatographyPreparative chromatography equipment is used for purification purposes, separating and collecting compounds eluted from a mixture which can then be subsequently used for further purposes. This can be done in large quantities or small. Preparative chromatography systems are usually low to medium pressure liquid chromatographs that are semi-automated. Explore a range of preparative columns and fraction collectors. Find the best preparative chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.TherapeuticsTherapeutics are treatments designed to alleviate or cure diseases. These include pharmaceuticals, biologics, and gene therapies, which work by targeting specific disease mechanisms. Advances in personalized medicine and biologics offer new hope for patients with conditions that were previously difficult to treat. Browse our peer-reviewed product directory to find therapeutic solutions for various diseases; compare products, check user reviews, and get pricing directly from manufacturers.Purification Solutions