ResourceLife Sciences
Purification-Free Labeling in Whole Cell Lysate and Binding Characterization by MST
4 May 2021For researchers performing biophysical analysis of proteins, a common hurdle encountered is having sufficient amounts of materials with the appropriate purity to perform detailed analysis. Here we demonstrate the utility of a RED-tris-NTA dye from NanoTemper Technologies that can be used to specifically label His-tagged proteins for MicroScale Thermophoresis (MST) binding studies directly in cell lysates.
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Monolith His-Tag Labeling Kit RED-tris-NTA
NanoTemper TechnologiesHis-Tag specific labeling for MST using RED-tris-NTA
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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LabelingMicroscale Thermophoresis