ResourceLife Sciences
Purification and Evaluation of Total RNA from Porcine Skin
5 Oct 2017By understanding the levels of RNA found in cells, scientists can better understand gene regulation and expression. Extraction of high quality RNA is the first and most vital step in performing many molecular biological techniques. RNA degrades relatively quickly so it's important to have a method that will isolate purified RNA while also minimizing degradation. In this application note, the potential of the Bead Ruptor Elite is evaluated for the extaction of RNA from porcine skin.
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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. 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