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Protocol for the Verification of Human Stem Cell Pluripotency

Bio-Techne
12 Nov 2012

Confirmation of pluripotency is a critical experimental step when studying human embryonic and/or induced pluripotent stem cells. This application note from R&D Systems presents a quick and simple protocol to confirm the ability of your starting cell population to differentiate towards ectoderm, mesoderm and endoderm lineages. Stem cell research studies most often require weeks of cell culture before an experimental hypothesis can be made. The ability to verify pluripotency at an early stage using the Human Pluripotent Stem Cell Functional Identification Kit provides valuable insight that may prevent weeks of wasted effort and reagents.

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Stem Cell Kits and Panels

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R&D Stem Cell Kits and Panels Research in developmental biology has led to the discovery of several types of stem cells, including embryonic stem (ES) cells, embryonic germ cells, and adult stem cells. Techniques and reagents developed for the in vitro culture, manipulation and characterization of stem cells provide unprecedented opportunities to study embryology and develop disease treatments. R&D Systems offers the following kits and panels for stem cell research: • R&D Differentiation Kits • R&D Expansion Kits • R&D Identification Kits • R&D Stem Cell Antibody Panels • R&D Stem Cell Antibody Arrays • R&D Stem Cell Primer Pair Panels • R&D Toxicity Kits • R&D Cytokine Panels for Hematopoietic Stem Cell Expansion • R&D Lineage Depletion Antibodies & Kits R&D Human/Mouse Dopaminergic Neuron Differentiation Kit R&D Mouse Oligodendrocyte Differentiation Kit R&D Neural Stem Cell Expansion Kits Human/Mouse/Rat Neural Stem Cell Expansion Kit - MonolayerR&D Human/Mouse/Rat Neural Stem Cell Expansion Kit - Neurosphere R&D Mesenchymal Stem Cell Functional Identification Kit R&D Human Mesenchymal Stem Cell Functional IdentificationR&D Mouse Mesenchymal Stem Cell Functional Idenitification KitR&D Neural Stem Cell Functional Identification Kits R&D Human Neural Stem Cell Functional Identification KitR&D Mouse/Rat Neural Stem Cell Functional Identification Kit R&D Stem Cell Antibody Panels - These panels are designed for analyzing the differentiation status of human ES cells by evaluating ES cell marker expressions. Each panel contains a group of antibodies that are recognized as markers for undifferentiated human ES cells. R&D Human Embryonic Stem Cell Marker Antibody PanelR&D Human Embryonic Stem Cell Marker Antibody Panel Plus R&D Stem Cell Antibody Arrays R&D Proteome Profiler Human Pluripotent Stem Cell Array- The Proteome Profiler Human Pluripotent Stem Cell Antibody Array can be used to detect the relative levels of expression of 15 different stem cell markers. R&D Stem Cell Primer Pair Panels The Human and Mouse/Rat Pluripotent Stem Cell Assessment Primer Pair Panels (Catalog #’s SC012 and SC015 respectively) profile the mRNA transcripts of fourteen genes that are frequently used as markers for molecular characterization of germ cells, ectodermal, endodermal, and mesodermal lineage-committed and undifferentiated embryonic stem cells. R&D Toxicity Kits The Neural Toxicity Assay kit contains specially formulated media supplements for the maintenance and expansion of neural precursors and for the differentiation of neural precursors into neurons. R&D Neural Toxicity Assay Kits R&D Cytokine Panel for Hematopoietic Stem Cell Expansion R&D Mouse Hematopoietic Stem Cell Expansion Cytokine Panel R&D Lineage Depletion Antibodies and Kits - lineage depletion antibodies and kits can be used to enrich for uncommitted cell populationsR&D Mouse Lineage Depletion Panel R&D MagCellect™ Mouse Hematopoietic Cell Lineage Depletion Kit - Is designed for the enrichment of hematopoietic non-lineage committed cells (lineage negative) from mouse bone marrow. For more information please press on the button that corresponds with your request that can be found in the top right hand side corner.

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Protocol for the Verification of Human Stem Cell Pluripotency