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Protocol for conjugating NHS-ester modifications to amino-labeled oligonucleotides
31 Aug 2020In this application note, MilliporeSigma describes the protocol for conjugating NHS-ester modifications, such as Texas Red®, to an oligonucleotide with an amino label, such as 5'-Amino-Modifier C6.
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OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.Conjugation