ResourceLife Sciences
Proteins Extraction from Eye Tissues with 96 Well-Plate Kits and Precellys® Evolution
11 Jun 2017In research on ophthalmology, mice are commonly used as models for retinal degeneration. Proteins expression analysis is needed to evaluate therapeutic agent efficiency, however, concentrated protein extracts are difficult to obtain because of the small size of tissues. Current methods extract proteins with a motorized pestle mixer, taking time and variability in extraction yield. The goal of this study was to prepare a large number of sample with high reproducibility, using a faster and easier methods.
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Precellys® lysing kits
Bertin TechnologiesA comprehensive range of beads and tubes for sample preparation
Precellys® Evolution - Tissue Homogenizer
Bertin TechnologiesThis product has been discontinued. See the newer version of this product linked below.
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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Homogenizers and BlendersHomogenizers and blenders are used to homogenize various types of material, including tissue, cells, plants, food, soil, biological and chemical samples for subsequent analysis. Lab equipment for sample homogenization includes handheld and benchtop homogenizers, bead mill, ultrasonic and multiple sample homogenizers, high shear homogenizers and blenders. Homogenizer bead and probe multipacks are available to avoid cross contamination of samples, as well as cooling systems for homogenizing temperature-sensitive samples. Homogenizers are often used in the first step of extraction of DNA, RNA and proteins from tissue and cell samples. Find the best homogenizers and blenders in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.homogenization