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Protein Separation with pH Gradients Using Composite Buffer Systems Calculated by the Agilent Buffer Advisor Software
6 Aug 2020This Technical Overview by Agilent provides some tips and tricks on how to correctly apply the Agilent Buffer Advisor Software for the generation of pH gradients over a wide range
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Agilent 1260 Infinity II Binary Pump
Agilent TechnologiesThe 1260 Infinity II Bio-Inert Pump is a quaternary UHPLC pump that is made of bio-inert material for use in biological and extreme pH applications. This pump enables gradient formation and solvent blending from up to four solvents and operates at pressures up to 600 bar and flow rates up to 10 mL/min.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HPLC Method DevelopmentIt is important to develop and validate HPLC methods in order to identify, quantify or purify compounds of interest in the most efficient way. Effective method development ensures optimization, quality control and cost effectiveness.