Protein Sample Preparation in Eppendorf Tubes® 5.0 mL

10 Feb 2015

Analysis of proteins from cultured cells often requires sample processing at a larger volume scale. Examples include isolation of cell fractions from larger homogenates, determination of enzyme activities as well as harvesting of proteins from sparsely growing cells. This application note investigates the performance of the Eppendorf 5.0 mL tube format with a comparative analysis for isolation and preparation of protein samples using different tube volume formats.

Eppendorf Tubes® 5.0 mL

Eppendorf

Eppendorf Tubes are the benchmark for simple and safe sample preparation from 0.5 to 2.0 mL. For larger volumes, 15 mL and 50 mL conical tubes are available. With medium sample volumes you may face an issue: they need to be processed with large conical screw cap tubes - impractical, inconvenient and often prone to contamination. Eppendorf now offers the "missing link": the Eppendorf Tube 5.0 mL. This product fills the gap between existing tube versions and enables the simple and safe processing of sample volumes up to 5.0 mL!Eppendorf Tubes® 5.0 mL Features: Ergonomic operation - Simple, practical and ergonomic single-hand operation. Large labeling area. Lid design - Hinged lid for minimized sample evaporation during storage and incubation in a wide range of temperatures from -86 °C to 80 °C. Material - Exceptionally high-quality, transparent polypropylene, free of plasticizers, biocides or mold release agents, for reliable test results. Certified purity - Available in lot-tested and certified Eppendorf Quality, PCR clean, Sterile and Eppendorf Biopur® purity levels. Centrifugation safety - Maximum safety and stability for centrifugation up to 25,000 × g and fast and efficient protocols. Conical shape - Compatible with accessories for conical 15 mL tubes - many existing adapters and racks can be used. Sample recovery - Available in Eppendorf LoBind® material for maximum recovery of valuable samples. Applications: Easier processing of samples up to 5.0 mL Higher yields in DNA isolation, especially with samples with limited quantity of source material Convenient and safe cell culture applications Easier sample access lowers the risk of contamination Preparation of mastermixes and buffers Less storage space needed Safe cell and tissue lysis

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Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.