ResourceLife Sciences
Protein gel electrophoresis handbook
27 Jun 2021Protein gel electrophoresis is a simple way to separate proteins prior to downstream detection or analysis and is a critical step in most workflows that isolate, identify, and characterize proteins. Download this handbook to find information on selecting the appropriate separation scheme, choosing the right gel equipment, and preparing samples for analysis.
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Invitrogen™ Bolt™ Bis-Tris Plus Gels
Thermo Fisher ScientificBolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions.
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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BlottingElectrophoresis