ResourceLife Sciences
Proof of Concept for the Quantitation of Peptides in Human Plasma by Liquid Chromatography-Multiple Reaction Monitoring (LC-MRM)
19 Jun 2012This work describes “proof of concept” data supporting sensitive and reproducible quantitative measurements of peptides in human plasma by multiple reaction monitoring (MRM) coupled with stable isotope dilution (SID)-MS. To demonstrate the specificity, sensitivity, and reproducibility of an LC-MRM assay, peptides and internal standards were spiked into human serum albumin and IgG depleted plasma.
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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Human PlasmaHuman plasma is the liquid component of blood, which carries blood components - nutrients, hormones, proteins, and waste - throughout the body. Plasma-based studies provide insights into biomarkers, immune responses, and disease mechanisms. Compare plasma handling tools and systems in our peer-reviewed product directory.Stable Isotope DilutionMRM