ResourceSeparations
Processing of a Complex Lipid Dataset for the NIST Inter-laboratory Comparison Exercise for Lipidomics Measurements in Human Serum and Plasma
Processing of a Complex Lipid Dataset for the NIST Inter-laboratory Comparison Exercise for Lipidomics Measurements in Human Serum and Plasma
8 Jun 2015This application note shows how an entire untargeted data set from NIST human plasma and serum reference materials can be processed using LipidSearch software, providing simultaneous identification and quantitation of lipids. In this note, almost 1000 lipid species were identified and quantified.
Links
Tags
LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Molecular Recognition SoftwareMolecular recognition software is widely used to analyze DNA, RNA, proteins and chemicals. The software can be useful for graphical viewing, comparative analyses, high-throughput screening, genomics, proteomics and phylogenetics. Molecular recognition software uses bioinformatics tools and analyses such as BLAST searches and generates structural predictions, 3D structures and sequencing information.Discovery SoftwareComputational techniques used in both the chemistry and biology aspects of drug discovery, for data acquisition, processing and storage. Software is used for analysis of pathways, target identification, genomics, proteomics, modelling, lead identification, compound analysis and drug modelling among many others. Software PlatformsSoftware platforms are useful for various stages of laboratory experiments from data collection to data storage and processing. For instance lab software is available for system control, data management, data analysis and qualification / validation.LipidsLipids are a diverse group of organic compounds that are insoluble in water, including fats, oils, and hormones. They are critical for cellular structure and energy storage. Explore lipid analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Human SerumHuman serum is found in blood plasma. The protein is used in a variety of applications including tissue engineering, transplantation and cell therapy.LC-MSLiquid Chromatography-Mass Spectrometry (LCMS) is a powerful analytical technique that combines the separation power of liquid chromatography with the detection capabilities of mass spectrometry. It is widely used for qualitative and quantitative analysis of complex mixtures in pharmaceuticals, proteomics, and environmental studies. Browse our peer-reviewed product directory to find the best LCMS systems, compare products, check reviews, and get pricing directly from manufacturers.