ResourceSeparations
Principles and solutions of size exclusion chromatography
29 Jul 2025Size exclusion chromatography (SEC) is a highly versatile separation technique. It is well suited for biomolecules sensitive to changes in pH, concentration of metal ions or cofactors, or harsh environmental conditions. Separations can be performed in the presence of essential ions, cofactors, detergents, urea, or guanidine hydrochloride at high or low ionic strength. Furthermore, SEC can be performed within a wide temperature range covering the requirements of most experiments.
Explore the use of SEC for the purification and separation of biomolecules. Discover how you can optimize results focusing on SEC resins and find common applications and theoretical principles behind this technique.
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Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Biomolecules