ResourceSeparations
Principles and methods of ion exchange chromatography
23 Jul 2025Ion exchange chromatography (IEX) is a frequently used technique in the separation and purification of proteins, peptides, nucleic acids, and other charged biomolecules, offering high resolution and group separations with high loading capacity.
Discover how IEX is well suited for capture, intermediate purification, or polishing steps in a purification protocol, and uncover theoretical and practical principles, the chromatography media (resins) available and how to select them.
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Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomoleculesProtein Purification