Principle of mitochondrial toxicity assessment using Agilent Seahorse XF Solution

15 Mar 2023

Agilent Seahorse XF analyzers are used to study cellular metabolism in a diverse array of research areas, including assessment of drug-induced mitochondrial toxicity. In this white paper, Agilent outlines the approach and design of the Agilent Seahorse XF Mito Tox assay solution for the detection of mitochondrial toxicity using standardized parameters.

Agilent BioTek Gen5 Software for Imaging & Microscopy

Agilent Technologies

Agilent BioTek Gen5 Software for imaging and microscopy offers automated image capture, processing and analysis for a broad range of samples, from whole organism imaging to high magnification subcellular imaging. Raw data and images are transformed into meaningful results with powerful built-in capture, process and analysis tools. The automated process enables simple and straightforward imaging and analysis workflows.

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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.MitochondriaToxicityToxicity is defined as the degree to which a substance can harm humans or animals. It is essential to study the toxicity of pharmaceutical products during the development process so that optimum dosage can be established for the administration of the drug.
Principle of mitochondrial toxicity assessment using Agilent Seahorse XF Solution