PrimeSTAR® GXL DNA Polymerase: One Enzyme for Every Reaction

22 Sept 2016

This application note illustrates the exceptional performance and versatility of PrimeSTAR GXL polymerase for both standard reactions, and for amplification of target sequences that often presents a challenge for standard PCR enzymes.

PrimeSTAR® GXL DNA Polymerase

Takara Bio

PrimeSTAR GXL DNA Polymerase is a high fidelity polymerase that provides efficient PCR amplification even in challenging situations, such as when amplifiying GC-rich templates, when there is eXcess template in the reaction mixture, and when amplifying Long products up to 30 kb (GXL). PrimeSTAR GXL Polymerase is the most robust high fidelity polymerase available, routinely allowing amplification of PCR products that cannot be amplified by other enzymes. PrimeSTAR GXL DNA Polymerase saves time by minimizing optimization efforts, amplifying targets regardless of the conditions.The PrimeSTAR GXL kit includes buffer and dNTPs.PrimeSTAR GXL DNA Polymerase is a modified version of PrimeSTAR HS enzyme that includes an elongation factor. This combination enables unsurpassed processivity. PrimeSTAR GXL DNA Polymerase displays outstanding performance in reactions containing excessive nucleic acid. Extraneous DNA ordinarily inhibits amplification by conventional polymerases because the amount of effective polymerase is limited by nonspecific binding. Thanks to the superior processivity of PrimeSTAR GXL DNA Polymerase, inhibition by excessive nucleic acid is limited, resulting in a much higher PCR success rate.Features: Buffer and dNTPs are conveniently provided in the PrimeSTAR GXL kit Highest processivity of all commercially available high-fidelity DNA polymerases Antibody-mediated hot-start formulation Proven performance as reported in peer-reviewed literature Applications: Successful, robust and high fidelity PCR regardless of conditions Amplification of products up to 30 kb (human genomic DNA), 40 kb (lambda DNA) or 13.5 kb (human cDNA) Easy amplification of GC- or AT-rich templates and targets containing repeats High tolerance for GC-rich template, excess nucleic acid, and when amplifying long products

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.EnzymesEnzymes are macromolecular biological catalysts used across life science, pharmaceutical and clinical research. Various enzymes can be purchased for research applications including ligases, high fidelity polymerases, nuclease, protease, restriction enzymes, tissue dissociation enzymes and enzyme inhibitors. Find the best enzymes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA AmplificationDNA Amplification is a technique used to amplify a single or multiple copies of DNA or mRNA by orders of magnitude. The most common method employed is PCR, but other options exist that eliminate the need for thermocycling.
PrimeSTAR® GXL DNA Polymerase: One Enzyme for Every Reaction