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Preparation of 384-well qPCR Plates using the ACSIA PCR Edition, a PrimaDiag Automated Pipetting Platform

PrimaDiag
3 Jul 2016

This application note describes the validation, reliability and the reproducibility of a qPCR plate’s preparation by measuring the results of the subsequent qPCR analysis. Sample preparation was performed using an automated pipetting platform, the ACSIA PCR Edition. The results demonstrate the advantages over using a manual pipetting method, especially as all protocols can be easily programmed with the help of the PrimaController®II software.

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ACSIA Tips

PrimaDiag

PrimaDiag provides its own brand of tips “ACSIA Tips”. This tips are high quality and autoclavable and ensure a great pipetting quality for the pipettes or its platforms. Specially designed to meet the quality requirements of ACSIA line, are also compatible with many manual pipettes The ACSIA Tips are available in two packaging: Rack:96 tips per rack 10 racks per box Refill:96 tips per plate 5 plates per refill 10 refills per box

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ACSIA PCR Edition - Automated Pipetting Platform

PrimaDiag

If you meet these needs: PCR setup in 96 and 384-wells plate Real time PCR setup in 96 and 384-wells plate Distribution Tube to plate transfer Plate to plate transfer Serial dilutions Hit picking Concentration normalization Plate compression/decompression SNP detection HLA genotyping PrimaDiag offers ACSIA XS PCR Edition. With the aim to increase your productivity, avoid contamination and improve work efficiency, PrimaDiag designed ACSIA XS PCR Edition. It is a compact and robust automated pipetting platform allowing performing all your simple protocols requiring a liquid handling. For instance, ACSIA PCR Edition can process without any human intervention the complete PCR setup in 96- or 384-well plate with our software, the PrimaController®II.ACSIA PCR Edition allows processing your complete PCR/qPCR setup in just 5 minutes. Moreover, thanks to an innovative system for the selection of the wells, it brings a very high flexibility for the fast tuning of your protocols and PCR/qPCR setup. Our automate offers you, with our pipetting heads, a higher accuracy and reproducibility. The quality of your samples at the end of the process will be greater.ACSIA XS PCR Edition is available with 8 SBS positions and integrate an interchangeable 250µl 1-channel pipetting head. Its software, the PrimaController®II, allows thanks to its realistic and interactive view and its real time help to increase your efficiency by focusing on your own tasks. Features: Automated platform designed for the PCR/qPCR setup in 96- and 384-wells plate Interchangeable pipetting head within seconds High quality pipetting with a CV lower than 2% for 1µl and lower than 0.05% for 250µl High efficiency automated platform managed by the most innovative and smartest software Add our protective enclosure for a better safety during your process Fast return on investment

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample ManagementSample management systems include sample storage devices such as freezers and plate storers, sample environment enclosures and sample organization, retrieval and sorter systems. Useful system features include high-throughput, automation, robotic arms, automated liquid handling and associated database systems. Accessories in sample management include barcode scanners, heat sealers and tubes.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Robotic WorkstationsRobotic workstations are automated platforms enabling the user to perform high-throughput, multi-application processes, from sample preparation tasks such as PCR set-up, DNA purification and NGS library preparation, to integrated sample prep and analysis workstations. Automation makes processes consistent, fast, precise and fully walk-away. Find the best robotic workstations in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Software PlatformsSoftware platforms are useful for various stages of laboratory experiments from data collection to data storage and processing. For instance lab software is available for system control, data management, data analysis and qualification / validation.Plate ProcessingAutomated plate processing is used in microplate technologies for high-throughput, speed and efficiency. Plate processing systems include microplate handlers for moving plates, heat sealers, stackers and carousels for storage, retrieval and delivery, microplate washers and piercers. Useful features of plate processors include walk-away automation, compatibility and easy integration with microplate systems.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.qPCRQuantitative PCR (qPCR) is a molecular biology technique used to amplify and measure the amount of a specific DNA or RNA target. It plays a crucial role in gene expression analysis, pathogen detection, and quantifying DNA or RNA in various applications. Real-time PCR provides precise, quantitative results and is widely used in research, diagnostics, and clinical studies. Browse our peer-reviewed product directory to find the best qPCR solutions; compare products, check reviews, and get pricing directly from manufacturers.Liquid Handling WorkstationLiquid handling workstations automate the process of sample preparation, improving accuracy and efficiency. These systems are essential for high-throughput applications in biological, chemical, and pharmaceutical research, reducing human error and increasing reproducibility. Workstations come equipped with robotic arms, pipetting tools, and other accessories to streamline lab workflows. Explore liquid handling workstations in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Plate ProcessingHigh ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.DNA AmplificationDNA Amplification is a technique used to amplify a single or multiple copies of DNA or mRNA by orders of magnitude. The most common method employed is PCR, but other options exist that eliminate the need for thermocycling.Genome SequencingGenome sequencing involves determining the complete DNA sequence of an organism's genome. It provides crucial information about genetic variations, mutations, and diseases. Advances in sequencing technologies, such as next-generation sequencing (NGS), have accelerated research in genomics, diagnostics, and personalized medicine. Explore genome sequencing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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Preparation of 384-well qPCR Plates using the ACSIA PCR Edition, a PrimaDiag Automated Pipetting Platform