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Precise Evaluation of Plant RNA Extraction Methods with Automated RT-qPCR Assay

Gilson, Inc.
9 Mar 2015

In this application note, functional corn RNA was quantitated by RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System after two different extraction methods: Promega’s Maxwell® 16 LEV (low elution volume) Plant RNA Kit and a competitor spin column-based plant RNA extraction kit. To enhance the pipetting accuracy and consistency, an automated pipetting assistant, Gilson PIPETMAX® 268, was used to reduce pipet-ting errors inherent in manual methods.

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Gilson PIPETMAX

Gilson, Inc.

PIPETMAX® is an automated pipetting solution for the efficient processing of high-throughput biological assays. It will help you to improve on the accuracy, reproducibility, and consistency among all samples processed. Unlike traditional automation platforms, PIPETMAX comes in a benchtop size that easily fits into any lab.

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Precise Evaluation of Plant RNA Extraction Methods with Automated RT-qPCR Assay