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Practical recommendations for cryo-EM single particle analysis reconstruction

Thermo Fisher Scientific
22 Sept 2019

In this application note, Thermo Fisher Scientific details the main principles of image processing for single particle analysis (SPA) and details practical solutions to this rapidly evolving computational landscape. Multiple software packages have been suggested to combat the large data sets of electron microscopy images acquired and correlated during cryo-electron microscopy (cryo-EM) SPA.

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Krios G4 Cryo-TEM for Life Sciences

Thermo Fisher Scientific

The Thermo Scientific Krios G4 Cryo-Transmission Electron Microscope (Cryo-TEM) is a compact TEM. The instrument can be installed in labs with a ceiling height below 3.04 m (~10 ft), as the microscope height it below 3 meters. The Krios G4 Cryo-TEM has improved ergonomics for easier sample exchange. Data acquisition set up is easier and faster thanks to enhanced automation, systematic user guidance and advanced performance monitoring. Simultaneo

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Data AnalysisData analysis hardware and software is available to make data processing straight-forward yet powerful. Data software can be used for math and stats, technical graphing and image analysis. In addition, software is available for specific data analysis of electrophoresis, densitometry, ELISA and DNA sequencing.Electron MicroscopyElectron microscopes (EM) are used to create high-resolution images of samples at the nanoscale by means of an accelerated beam of electrons as a source of illumination. Types of electron microscope include scanning electron microscopes (SEM), transmission electron microscopes (TEM), scanning transmission electron microscopes (STEM) and cryo-electron microscopes. Focused ion beam (FIB) microscopes are useful for modifying or milling a sample surface with nanometer precision, as well as imaging. Find the best electron microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Particle CharacterizationParticle characterization instruments are used to determine particle size distribution, shape, surface area, zeta potential, density and porosity of particles and materials. Multiple tecchniques are available for determining particle size, shape and count including dynamic light scattering (DLS), laser diffraction, electrozone (Coulter technique), imaging particle analysis and single particle optical sensing. Determine the density of your material with a gas pycnometer or examine its surface area and porosity with gas adsorption analyzers and mercury porosimeters. Find the best particle characterization instruments in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Digital MicroscopyDigital microscopy involves using digital cameras and sensors to capture high-resolution images of samples for analysis. It offers enhanced imaging capabilities compared to traditional optical microscopy and is widely used in biological and material science research. Explore digital microscopy systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Image AnalysisImage analysis involves the extraction of meaningful information from images, often using software to quantify and interpret visual data. It is widely used in cell biology, material science, and diagnostics. Increasingly, AI is being used to streamline image analysis. Explore image analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Data AnalysisThe analysis of data is the process of transforming, modeling and evaluating data to discover useful information from experimental results. MicroscopyMicroscopy is a technique used to observe small objects in detail, from cells to materials, using light or electron microscopes. It enables researchers to examine structures with high resolution, aiding in fields such as biology, medicine, and materials science. With advanced microscopy techniques, scientists can gain insights into cellular processes, tissue structures, and material properties. Explore the best microscopy solutions in our peer-reviewed product directory, compare products, read customer reviews, and get pricing directly from manufacturers.Digital ImagingDigital imaging is a technique that captures images of biological and chemical samples in high resolution, often using advanced cameras and sensors. This technology is crucial in microscopy, diagnostics, and quality control. Explore digital imaging systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.electron microscopy

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Practical recommendations for cryo-EM single particle analysis reconstruction