Positive pressure-based plasmid preparation using QIAprep chemistries

29 Mar 2020

In this application note, Tecan describes a semi-automated protocol for the extraction of plasmid DNA using QIAprep 96 Turbo and QIAprep 96 Plus plasmid kits on a Resolvex A200 positive pressure workstation. This application note highlights the highly efficient extraction of plasmid DNA for pharma and life sciences applications while using low pressure profiles for filtration, washing and elution steps, and fast buffer exchange.

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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.DNA ExtractionDNA extraction is a critical step in many biological studies, including genomics, diagnostics, and forensic analysis. The process involves isolating DNA from cells or tissues, and the quality and purity of the extracted DNA can impact downstream applications. Explore DNA extraction products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.
Positive pressure-based plasmid preparation using QIAprep chemistries