ResourceLife Sciences
PhyTip® ProPlus Affinity Columns for Antibody Purification
13 Feb 2013The PhyNexus PhyTip® columns are purification tools that radically simplify the capture, purification and enrichment of proteins from a variety of sources. This application note demonstrates how the PhyTip ProPlus columns are ideal for antibody screening from hybridoma supernatants or for high throughput extraction and purification of expressed antibodies.
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PhyTip® Columns and MiniPrep PhyTip® Columns
BiotagePhyTip® columns use dual-flow chromatography within pipette tips to automate purification workflows. This technology delivers high reproducibility and exceptional sample purity for efficient, reliable results.
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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Antibody Purification